Objective:To investigate the effects of eight-treasure powder on the improvement of spleen and stomach functions.Methods:3 dpf(3 days post-fertilization)zebrafish are randomly selected in a dynamic manner to construct a gastrointestinal mucosal injury model by giving tritrobenzene sulfonate in a water-soluble manner.After successful modeling,eight-treasure powder and prednisolone are given in water solution,respectively.The repair effect of eight-treasure powder on gastrointestinal mucosal injury is evaluated by analyzing the number of intestinal cup-cells in zebrafish stained with Alcian blue.5 dpf(5 days post-fertilization)zebrafish are randomly selected dynamically and given aluminum sulfate in water solution to establish a constipation model.After successful modeling,eight-treasure powder and domperidone are given in the way of water solution,and the improvement effect of eight-treasure powder on constipation is evaluated by analyzing the intestinal fluorescence intensity of the zebrafish.Result:1 000,2 000 μg·mL-1 eight-treasure powder significantly improve the gastrointestinal mucosal injury of zebrafish,and show dose correlation.500,1 000,2 000 μg·mL-1 eight-treasure powder has a significant effect on zebrafish constipation,and it is dose-dependent.Conclusion:Eight-treasure powder can improve the spleen and stomach of zebrafish to some extent.
维普
万方数据
