Determination the Content of Patulin in Fruit Juices by Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Mass Spectrometry-Isotope Dilution Method
Determination the Content of Patulin in Fruit Juices by Solid Phase Extraction-Ultra Performance Liquid Chromatography Tandem Mass Spectrometry-Isotope Dilution Method
The determination of patulin in fruit juices by solid phase extraction-ultra performance liquid chromatography tandem mass spectrometry-isotope dilution method was presented.After preparation and extraction,the samples were purified by solid phase extraction column,separated by Athena UPLC C18 column,eluted with acetonitrile and water as mobile phase.Electrospray ion source(ESI-),multiple reaction monitoring mode analysis,internal standard assay.The results showed that the mass concentration of picillin showed a good linear relationship in the range of 0 ng·mL-1 to 250 ng·mL-1,correlation coefficient r2 was more than 0.999,and the limit of quantification was 2.5 μg·kg-1.The recoveries were 83.18％to 99.25％at the low,middle and high levels(10 μg·kg-1,50 μg·kg-1 and 250 μg·kg-1).The method is simple,rapid,reproducible and suitable for the determination of penicillin in fruit juice drinks.
关键词
展青霉素/果汁饮料/固相萃取-超高效液相色谱串联质谱-同位素稀释法
Key words
patulin/fruit juices/solid phase extraction-ultra performance liquid chromatography tandem mass spectrometry-isotope dilution method
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