Objective:To establish a method for simultaneous determination of 3 phenolic acids and 3 flavonoids in Lycium barbarum L.by HPLC.Method:C18 column was used,acetonitrile-0.5％acetic acid solution was used as mobile phase,gradient elution,UV detector,flow rate was 1.0 mL·min-1,column temperature was 25 ℃,detection wavelength was 320 nm,sample size was 10 μL.Result:The 6 compounds had a good linear relationship in their linear range,the relative standard deviations were less than 5％,and the recoveries were 99.7％to 106.9％.Conclusion:The method is rapid,simple,accurate and reproductively suitable for the determination of flavonoids and phenolic acids in Lycium barbarum L..
关键词
高效液相色谱法/枸杞/黄酮
Key words
high performance liquid chromatography/Lycium barbarum L./flavone
维普
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