Optimization of the Process of Extracting Total Flavonoids from Seed Meal of Hippophae rhamnoides L.with Eutectic Solvent
Using seed meal of Hippophae rhamnoides L.as raw material and flavonoid extraction as the investigation index,the process parameters of total flavonoids extracted from seed meal of Hippophae rhamnoides L.from deep eutectic solvents(DES)were optimized by single factor and response surface experiments.The results showed that the optimal extraction process was as follows:the molar ratio of choline chloride to glucose was 4∶1,the water content of DES was 50%,the ratio of solid-liquid was 1∶23,the extraction temperature was 60℃,and the extraction time was 60 min.Under these conditions,the total flavonoids extracted from seed meal of Hippophae rhamnoides L.reached 23.93 mg·g-1.
deep eutectic solventsHippophae rhamnoides L.seed mealtotal flavonoids