Identification of Wx Genotypes in Excellent Breeding Materials of Rice(Oryza Sativa L.)and Analysis of Related Traits
In order to identify the Wx genotype and the phenotype of its related traits in the elite breeding materials,the(CT)n sequence upstream of the first intron of the Wx gene,the first intron+1 G/T SNP,the A/G SNP of exon 4 and the A/C SNP of exon 6 of 27 elite rice breeding materials were identified by DNA molecular markers combined with specific primer am-plification fragment sequencing.Amylose content(AC)and gel consistency(GC)were also measured.The results showed that there were five(CT)n polymorphisms(n=10,11,12,17,18)upstream of the first intron of the Wx gene in the 27 rice materials.Six of them were of the Wxa genotype,16 were of the Wxb genotype,three were of the Wxhp genotype,one was of the Wxin genotype,and one was wx.Wxa materials had higher AC(23.1%～29.0%),Wxb materials had moderate AC(10.9%～16.4%),Wxhp materials had lower AC(5.7%～9.8%),Wxin materials had an AC of 18.4%,and wx materials were glutinous rice with an AC of 3.5%.(CT)n polymorphisms and G/T SNP explained 88.9%and 85.2%of the variation in amylose content,respectively,and(CT)n polymorphism markers had high selection accuracy.There was a significant negative correlation between AC and GC(r=-0.4827*),but there were also specific materials with high AC and high GC,or low AC and low GC.This study provides material and technical references for cultivating excellent hybrid rice parents,breeding high-quality and special hybrid rice varieties.
万方数据
维普
