Screening and Identification of Genes Contributes to Tolerance Inhibitors Derived from Lignocellulose Hydrolysis
In recent years,lignocellulose,represented by bamboo chips and straw,has been widely applied to produce bioethanol.However,a series of toxic inhibitors derived from the hydrolysis process will inhibit the fermentation of ethanol in Saccharomyces cerevisiae.To improve the fermentation efficiency under the stress of toxic inhibitors without increasing the pro-duction cost,it is the key to screen the genes in S.cerevisiae that contribute to tolerance these inhibitors in a broad range.Due to whole-genome knockout library scanning,bioinformatics analysis and gene complement verification,this study screened the com-mon genes,namely ASC1,ISC1,DRS2,RIC1,RPE1,YPT6,SEC66 and ERG2,in S.cerevisiae that contribute to tolerance the representative inhibitors,such as furfural,phenol,acetic acid and their mixed inhibitors.According to function analysis of genes,it is identified that the proteins in transcription regulation,signal transduction,oxidative stress tolerance,ergosterol biosynthesis,au-tophagy and endocytosis are related to the tolerance of complex inhibitors derived from lignocellulose hydrolysate.It has important reference significance for transformation of the engineering strain for bioethanol fermentation.
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