Determination of lactic acid in fermented feed by high performance liquid chromatography
A liquid chromatography method was developed for the accurate determination of lactic acid content in fermented feed.Using C18 column and UV detector,lactic acid could be well separated in 10 minutes.The mobile phase was potassium dihydrogen phosphate solution,the detection wavelength was 210 nm.The external standard method was used for quantitative determination.The method had a good linearity in the concentration range of 1 mg/L to 300 mg/L,R2＞0.999.The limit of de-tection was 30 mg/kg,the limit of quantitation was 50 mg/kg and the recovery rate of standard addition were 86.6％-98.0％.This method had high sensitivity,accuracy and good repeatability,and was suitable for the determination of lactic acid in fer-mented feed.
万方数据
维普
