粮食与饲料工业2024,Issue(1) :70-71.DOI:10.7633/j.issn.1003-6202.2024.01.015

核酸提取试剂盒对饲料中牛羊源性成分检测的影响

The effect of nucleic acid extraction kit on the detection of bovine and sheep derived components in feed

李泰 朱英才 骆璐 彭强 凌洪权 廖星杰 王钦 张承双
粮食与饲料工业2024,Issue(1) :70-71.DOI:10.7633/j.issn.1003-6202.2024.01.015

核酸提取试剂盒对饲料中牛羊源性成分检测的影响

The effect of nucleic acid extraction kit on the detection of bovine and sheep derived components in feed

李泰 1朱英才 1骆璐 1彭强 1凌洪权 1廖星杰 1王钦 1张承双1
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作者信息

  • 1. 重庆市动物疫病预防控制中心,重庆 401120
  • 折叠

摘要

实时荧光聚合酶链反应法(荧光PCR法)是目前饲料中牛羊源性成分的定性检测中常用方法之一.为了熟练操作和掌握这个方法,使得实验室工作人员在做饲料中牛羊源性成分定性这类检测时能有一个清晰的认识,通过核酸提取试剂盒的选取及平行实验比对,对此进行了阐释.

Abstract

Real-time fluorescent polymerase Chain reaction is one of the commonly used methods for qualitative detection of cattle and sheep derived ingredients in feeds.In order to proficiently operate this method,and enable laboratory staff to have a clear understanding of qualitative testing of bovine and sheep derived components in feed,this experiment explains this through the selection of nucleic acid extraction kits and parallel experimental comparisons.

关键词

荧光PCR法/牛羊源性成分/检测分析/核酸提取试剂盒

Key words

fluorescent PCR method/bovine and sheep derived components/detection and analysis/nucleic acid extraction kit

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出版年

2024
粮食与饲料工业
国家粮食储备局 武汉科学研究设计院

粮食与饲料工业

CSTPCD
影响因子:0.513
ISSN:1003-6202
参考文献量7
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