To improve the cholesterol-lowering effect of cereal dietary fiber(DF),this study adopts Neurospora crassa to modify the black rice DF and then holds a discussion of the fermentation process as well as functional properties of fermenta-tion-modified DF.The results indicate fermentation-modified black rice DF appears to have the most active cellulase and best fermentation effect in the following conditions:1∶0.7 material-to-liquid ratio,29℃ fermentation temperature,132h fermenta-tion duration.Compared with non-fermented black rice DF,the fermented one has 6.56 mL/g water retention rate and 5.03 g/g water absorption expansion ratio.According to the results,the fermentation-modified black rice DF could absorb cholesterol at a ratio of 51.24 mg/g in simulated intragastric environment and 58.76 mg/g in simulated small intestinal environment,being twice more efficient than the non-fermented one.Animal experiments suggest fermentation-modified black rice DF manifests sat-isfactory cholesterol-lowering effect.To sum up,the fermentation-modified black rice DF has beneficial functional properties and cholesterol-lowering effect,which may offer some reference for future nutrition research.
关键词
黑米/膳食纤维/料液比/发酵温度/发酵时间/纤维素酶活力/胆固醇吸附能力
Key words
black rice/dietary fiber/material-to-liquid ratio/fermentation temperature/fermentation time/cellulase activity/cholesterol adsorption capacity