Effects of mannan-oligosaccharide(MOS)on production performance,egg quality,immune function and duo-denum morphology of laying hens in the later stage of laying
The aim of this study is to evaluate the effects of MOS on production performance,egg quality,immune function,and duodenum morphology of laying hens in the later stage of laying.A total of 240 65-week-old Hy-Line variety brown hens were divided into four groups with six replicates in each group and 10 in each replicate.The research objects underwent a 14-day pre-trial and a 56-day formal trial period.During the pre-trial,all hens were fed basic ration;while in the trial,basic ration was supplemented with different concentrations of MOS(0 g/kg,0.2 g/kg,0.4 g/kg,and 0.6 g/kg).Results indicated that:(1)the group added with 0.6 g/kg MOS in the basic ration witnessed a significantly higher laying rate(P<0.05)when compared to the control group(0 g/kg);(2)addition of MOS in the basic ration at 0.4 g/kg and 0.6 g/kg could significantly improve yolk color and shell thickness(P<0.05);(3)the 0.6 g/kg MOS level significantly raised serum albumin(P<0.05),and adding MOS in the feed reduced the low-density lipoprotein but raised the high-density lipoprotein level in the serum of the lay-ing hens(P<0.05);(4)addition of MOS in the basic ration significantly enhanced serum IgA,IgM,IgG,TNF-α,and IL-6 lev-els(P<0.05);and(5)addition of MOS in the basic ration significantly increased villus height in the duodenum(P<0.05),with 0.4 g/kg and 0.6 g/kg MOS improving the villus-to-crypt depth ratio(P<0.05).In conclusion,MOS added in the feed could effectively improve the production performance and immune status of hens in later-stage laying and contribute to bringing in better duodenum morphology.Under the experimental conditions,the optimal MOS inclusion level in the ration for late-phase laying hens was determined to be 0.4 g/kg.
later stage of layinglaying hensproduction performanceegg qualityimmune functionduodenum morphology