Rapid determination of chloramphenicol drugs in feeds with pass-through solid phase extraction column and ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)
Rapid determination of chloramphenicol drugs in feeds with pass-through solid phase extraction column and ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS)
This study aimed to establish an approach for rapidly detecting and analyzing chloramphenicol drugs in feeds by combing Prime HLB fast-purifying pretreatment technology with ultra performance liquid chromatography tandem mass spec-trometry(UPLC-MS/MS).Extracted with acetonitrile,feed samples went through further purification in Prime HLB solid-phase extraction column,gradient elution with acetonitrile-water as mobile phase in the monitoring mode of UPLC-MS/MS MRM and analytical test in ESI mode.The substrate was quantified using internal standard method.Under fully optimized pre-treatment conditions and GC/MS(gas chromatography-mass spectrometry)conditions,chloramphenicol drugs presented good linearity(r＞0.999 0)within the mass concentration of 0.5-10.0 μg/L.The detection limit was 0.1 μg/kg,while the quanti-fication limit was 0.5 μg/kg.The average recovery rate ranged from 93.1％to 103.2％,and the relative standard deviation(n=5)was no higher than 3.1％when samples were added at 1.0 μg/kg,2.5 μg/kg,and 5.0 μg/kg.The method features simple pretreatment procedures,fast analysis,and high precision,which proves its applicability to screening and quantitative a-nalysis of chloramphenicol drugs in feeds.
关键词
Prime/HLB/UPLC-MS/MS/氯霉素类/饲料检测
Key words
Prime HLB/UPLC-MS/MS/chloramphenicol drugs/feeds detection
维普
万方数据