With feeding acidifier as test sample in a series of trials,this study reported the development of a novel HPLC-based analytic method to determine 8 organic acids in feed acidifier.For this novel method,CAPCELL PAK MGⅡ C18 chroma-tographic column was used,the mobile phase consisted of 0.1％phosphoric acid diluent and acetonitrile(at a ratio of 98:2,V/V),the detection wavelength was 210 nm,the flow rate was 0.8 mL/min,the column temperature was 30℃,and quantifi-cation was done via external standard method.The findings indicated all 8 organic acids in the feeding acidifier sample could be well separated within their respective linear ranges.Their linear range was 0.001 6-4.780 8 mg/mL,while their detection limit range was 0.002-0.525 mg/mL.The correlation coefficient was i0.999 9.The developed method proved to be a sensi-tive and accurate method in detecting the 8 organic acids with satisfactory repeatability.
维普
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