Objective To investigate the clinical value of 3DSlicer multi-modal fusion 3D visualization technology in assisting the surgical treatment of limbic system glioma.Methods The clinical data of 67 cases of limbic glioma treated in our hospital were retrospectively analyzed and divided into 32 cases of 3DSlicer reconstruction group(experimental group)and 35 cases of tradi-tional operation group(control group)according to surgical methods.The differences of cognitive function(MMSE)score,Karnofsky performance status(KPS),tumor resection degree,complica-tions and other clinical effects between the experimental group and the control group were ana-lyzed.Results The MMSE score of cognitive function in the experimental group(28.50±2.20)was higher than that in the control group(25.40±4.972),P=0.002,the difference was signifi-cant.One month after operation,the KPS score of the experimental group(94.53±7.112)was higher than that of the control group(84.86±10.396),with significant difference(P<0.001).The total resection rate of the experimental group was higher than that of the control group(Z=-3.271,P<0.001).The complication rate of the control group was higher than that of the ex-perimental group.Conclusion 3DSlicer multi-modal fusion 3D visualization technology can guide the surgical approach,maximize the safe resection of limbo system glioma,reduce postopera-tive cognitive and neurological dysfunction,reduce related complications,and increase the quality of life of patients.