On Ultrasound Extraction and Content Determination of Total Flavonoids in Ginkgo biloba
The study used Ginkgo biloba leaves and Ginkgo biloba fruits as raw materials to extract the total flavonoids of Ginkgo biloba by ultrasound.The content of total flavonoids in different parts of ginkgo biloba was determined by uv-Spectrophotometer under single-factor experimental conditions,to search for the best technological conditions of extracting flavonoids from Ginkgo biloba leaves and Ginkgo biloba fruit.The optimum conditions of ultrasonic extraction of flavonoids from Ginkgo biloba leaves were as follows:ethanol concentration 60%,solid-liquid ratio 1∶40(g/mL),particle size 80 mesh,extraction time 60 min,extraction temperature 70 ℃,under these conditions,the extraction rate of flavonoids from Ginkgo biloba leaves was 2.44%,and the optimum conditions for ultrasonic extraction of flavonoids from Ginkgo biloba fruits were as follows:ethanol concentration 60%,ratio of solid to liquid 1∶40(g/mL),particle size 45 mesh,extraction time 75 min,the extraction temperature was 60 ℃,and the extraction rate was 0.83%.Under the optimum extraction conditions,the extraction rate of flavonoids in Ginkgo biloba leaves and ginkgo biloba fruit was relatively stable,and the content of flavonoids in Ginkgo biloba leaves was significantly higher than that in Ginkgo biloba fruit.