New fractionation technology of cottonsee d oil was investigated in order to solve problems in traditional fraction-ation of cottonseed oil, which were long crystallization time and large energy consumption. Combined pre-crystallization process by adding a high melting point seed with conventional fractionation of process palm oil, tnew technology was improved in this re-search. Tests showed that liquid oil extracted fat was still clear and transparent after being frozen l0d at 0℃, and time of freezing test could up to 24.5 h, which satisfied requirements of A-level cottonseed freezing tests. Production costs could be reduced by improving processes of existing fractionation of cottonseed oil, and this technology has a very important significance in improving utilization of cottonseed oil and relieving shortage situation of oil in China.