Ultrasonics-assisted alkaline method has been employed into the process of extraction of hemicelluloses A from distillers grains. Influences of KOH mass concentration, extraction temperature and ultrasonic time were evaluated; the best con-dition of extraction was optimized by response surface methodology ( RSM) . Results showed that optimal extraction conditions were KOH mass concentration of 7%, extraction temperature of 58℃, and ultrasonic time of 21 min. Under this condition, ex-traction rate of hemincellulose A was 37.17%.