Through studying effects of glucose acid conversion rate and glucose utilization rate on different furfural concen-tration, tolerance of Rhizopus oryzae LS-1 on furfural inhibitor in lignocelluloses hydrolysates were explored, and tolerance con-centration was searched. Results showed that tolerance threshold for Rhizopus oryzae LS-1was 12.5g/L, when furfural was indi-vidually added. Yield of lactic acid increased when furfural concentration was lower than 12.5g/L. quantitative view to the lactic acid production under the inhibition of lignocellulose degradation products were gave, and it has some guiding significances for overcome the difficulties of the lignocellulosic hydrolysates fermentation.