Determined optimum fermentation medium of Pseudomonas sp. koreensis that carbon, nitrogen species were glucose and ammonium chloride by single factor test. At the same time, with changing concentration of each component in culture medium, test results showed that three factors of glucose, ammonium chloride and potassium hydrogen phosphate were major on PHB yield. Based on single factor test, using Design Expert8.0 to design Box-Behnken response response surface experimental with three factors and three levels, five central points in 17 groups, fit regression equation was get. Finally, determined optimum fermentation medium was glucose concentration 16.64g/L, chloride concentration 3.94g/L and potassium hydrogen phosphate concentration 1.45g/L, PHB production reached 3.18g/L, compared with before optimization increased by 1.8 times, effect was significant.
NETL
NSTL
万方数据
维普
