首页|响应曲面法优化茶皂素提纯工艺研究

响应曲面法优化茶皂素提纯工艺研究

Purification Process Optimization of Camellia Saponin by Response Surface Methodology

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本文主要以有机溶剂法浸提油茶籽外壳粉得到的粗提液作为原料,应用絮凝-沉淀法提纯皂素.在单因素试验的基础上,利用Mintab15.0软件得到回归方程的预测模型并进行响应面分析,确定利用絮凝-沉淀法提纯皂素的最优工艺条件.絮凝试验: 加入16%的壳聚糖溶液和皂素损失率为28.46%,杂质损失量为1.39%; 沉淀试验: 氧化钙加入量1.78%、沉淀温度32.75℃和沉淀时间3.01h,沉淀率为98.99%; 释放试验: 释放温度52.2℃、释放时间3.17h和碳酸氢铵加入量4.09%,释放率为3.61%.最后,在最优提纯工艺条件下测得纯度为60.4%.
In this paper, saponin was purified by flocculation-precipitation, and crude extraction from camellia oleifera seed shell was as raw material. On the basis of single factor experiments, purification process was optimized using response surface methodology, and a mathematical regression model was established by Mintab15.0 software. Optimal purification conditions obtained from this model were as follows: 16% Chitosan solution added in crude extraction; 1.78% CaO was added in supernatant after flocculation to subside for 3.01h at 32.75℃; 4.09% NH4HCO3 was added to the sediment, and then released at 52.2℃ for 3.17h. Under conditions, content of camellia saponin was 60.4% in the final.

response surface methodologysaponinflocculation-precipitationrelease

赖徐倩、仲山民、王超、郑剑、常银子

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浙江农林大学 农业与食品科学学院, 浙江 临安311300

响应曲面 皂素 絮凝-沉淀 释放

浙江省重点科技创新团队项目

2011R50030

2015

粮油加工(电子版)

粮油加工(电子版)

ISSN：
年,卷(期)：2015.(12)
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