Growth and Physiological Response of Triploid Ulmus pumila to NaCl Stress
In order to study the salt stress response characteristics of triploid Ulmus pumila,the triploid Ulmus pumila asexual lines were used as test material to determine the changes of growth,physiological and biochemical indexes in five different stress times(0 d,7,14,21 d and 28 d)under 0.9%NaCl treatment.The results showed that the plant height and relative tall growth of triploid Ulmus pumila were significantly higher than that of diploid Ulmus pumila at 0 d and 28 d of NaCl stress;with the extension of the salt stress time,the relative conductivity(REC)and malondialdehyde(MAD)content of Ulmus pumila of different ploidy increased gradually,and the net photosynthesis rate(Pn),transpiration rate(Tr),stomatai conductance(Gs),intercellular carbon dioxide concentration(Ci)and Chlorophyll(Chl)content gradually decreased,triploid Ulmus pumila leaves REC,MDA content,Pn,Tr,Gs,Ci,Chl changes more slowly than the diploid;different ploidy of Ulmus pumila superoxide dismutase(SOD),peroxidase(POD),catalase(CAT)with the prolongation of the time of salt stress showed a trend of rise and then decline,the time of the treatment of the triploid Ulmus pumila SOD,POD and CAT activities were higher than those of diploid Ulmus pumila;the soluble sugar(SS)content of different ploidy Ulmus pumila increased gradually with the extension of treatment time,the soluble protein(SP)content of diploid Ulmus pumila showed a trend of increasing and then decreasing with the extension of treatment time,while the SP content of triploid Ulmus pumila showed a trend of increasing gradually with the extension of treatment time,and the SS content and soluble protein content of triploid Ulmus pumila were higher than that of diploid Ulmus pumila with the extension of each treatment time.SP content was higher than that of diploid Ulmus pumila.The photosynthetic system of triploid Ulmus pumila was less damaged under NaCl stress,and the antioxidant capacity and cell stability were stronger than those of diploid Ulmus pumila,which had stronger salt tolerance.