Effect of Pressure-cutting Culture on Differentiation and Proliferation in Stem Tips of Acanthopanax senticosus
In order to clarify the effect of pressure-cutting culture on the proliferation of tube seedlings,we used axillary buds of Acanthopanax senticosus as the study material and screened the proliferation medium:MS+KT1.0 mg/L+6-BA1.5 mg/L+NAA0.2 mg/L.We found that a 4-fold increase in stem tip proliferation of tube seedlings,and led to a rapid growth of newly differentiated axillary buds,leaf unfolding and vigorous growth.This study facilitates the optimization of tube seedlings and provides a basis for rooting culture.
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