压切培养对刺五加茎尖分化增殖的作用
Effect of Pressure-cutting Culture on Differentiation and Proliferation in Stem Tips of Acanthopanax senticosus
田新华 1郭琪 1王福德 1李艳霞1
作者信息
- 1. 黑龙江省林业科学研究所,黑龙江 哈尔滨 150081
- 折叠
摘要
以刺五加腋芽为材料,研究了压切培养技术对试管苗增殖的作用,筛选出增殖培养基配方:MS+KT1.0 mg/L+6-BA1.5 mg/L+NAA0.2 mg/L.结合此技术诱导试管苗分化,增殖倍数达 4.0 倍,可使新分化的芽苗快速进入自主生长,叶片展开,生长势强,有利于培养试管壮苗,为生根培养奠定基础.
Abstract
In order to clarify the effect of pressure-cutting culture on the proliferation of tube seedlings,we used axillary buds of Acanthopanax senticosus as the study material and screened the proliferation medium:MS+KT1.0 mg/L+6-BA1.5 mg/L+NAA0.2 mg/L.We found that a 4-fold increase in stem tip proliferation of tube seedlings,and led to a rapid growth of newly differentiated axillary buds,leaf unfolding and vigorous growth.This study facilitates the optimization of tube seedlings and provides a basis for rooting culture.
关键词
刺五加/压切技术/增殖培养Key words
Acanthopanax senticosus/pressure-cutting culture/proliferation culture引用本文复制引用
出版年
2025
NETL
NSTL
万方数据