Cloning and Sequence Analysis of 1-Deoxy-D-Xylulose 5-Phosphate Gene cDNA from Eucommia ulmoides
Homologous DXR gene cDNA was isolated from leaves of Eucommia ulmoides by method of reverse transcription polymerase chain reaction ( RT-PCR ) and rapid amplification of cDNA ends ( RACE ) technique, and named after EuDXR. The nucleotide sequence of EuDXR was 1 814 bp and contains an open reading frame (ORF) of 1 437 bp encoding 478 amino acids with a molecular weight of about 51. 71 kD,and the theoretical isoelectric point of which was 5.79, representative motifs of plant DXR and 26 potential functional sites were deduced in the a-mino acids sequence of EuDXR. The results of phylogenetic analysis suggested that the protein sequence of EuDXR was more similar to that of Zea mays and Oryza saliva than other species, and followed by Hevea brasiliensis, Arabi-dopsis thaliana and Nicotiana tabacum.
NETL
NSTL
万方数据
维普
