Study on Cutting Propagation of Thuja sutchuenensis,An Endangered Species Endemic to China
The cutting propagation trial on Thuja sutchuenensis was carried out in a tree nursery located at Xianyi Village, Chengkou County, Chongqing, China and in the greenhouse of Chinese Academy of Forestry, Beijing. Through the experiment, the effects of various factors, including age of donor trees, kinds and concentrations of auxin, substrate and physically-root-promoting, on rooting were examined. The results are as follows: (1) The rooting rate of cuttings from one-year-old T. sutchuenensis sapling varied from 74. 76% to 96. 7% 120 days after cutting under different disposed conditions in the nursery of Xianyi, where adopting automatic spray facilities and medium with vermiculite: peat: pearlite (1:1:1, v/v/v). Specifically, the cuttings disposed to 2 000 mg·L-1 IB A showed the highest rooting rate, with a decreasing tendency in water, 1 000 mg ·L-1 IB A, and 1 000 mg·L-1 IB A plus 500 mg·L-1 NAA (P <0. 05). (2) In Beijing, where covered by plastic films and medium with vermiculite;peat; pearlite (1:1:1, v/v/v) , the one-year-old T. sutchuenensis cuttings showed a lower rooting rate than that in Xianyi (P <0. 05) , with a range from 25, 93% to 45. 37% 160 days after cutting. Moreover, the effect of disposing conditions on rooting rates in Beijing was different from that in Xianyi, with a lower rate in water and an equally high rate in 2 000 mg·L-1 IBA, and 1 000 mg·L-1 IBA plus 50 mg·L-1NAA. The obvious difference of rooting rate between Xianyi and Greenhouse may be aroused by nutrition of donor trees and the environmental factors. (3) For a-dult T. sutchuenensis cuttings In Xianyi, which were disposed to 27 combinations of different kinds of hormone and solutions of physically promoting root, the highest rooting rate was only 20. 83% . The combination, under which the rooting rate was higher than 10% , is the combination of water-disposed plus with substrate of vermiculite: peat: pearlite (1:1:1, v/v/v) , that of disposing to 1 000 mg ·L-1 IBA plus 500 mg·L-1 NAA and plus a substrate with peat:pearlit (2: 1, v/v) , and that of 1 000 mg·L-1 IBA disposed with an even substrate of vermiculite; peat: pearlite (1:1: 1, v/v/v) and plus washing through flowing water.
Thuja sutchuenensiscutting propagationrooting rateauxin type