Geographic Variation of Morphological Characters among Cultivated Neem (Azadirachta indica) Populations in Yunnan, China
By means of morphological markers with completely random design and nest design,the geographic variation pattern of the morphological characters of neem (Azadirachta indica) was revealed.Sampled at level of 90 individuals,9 populations,14 phenotypes along gradients of latitude and altitude,and analyzed by multivariate statistics,the results are as follows:The variations existed both among populations and within population.The nutrient characters were positively correlated to the annual mean temperature (21.7-24.8 ℃),and negatively correlated to altitude (197.0-1277.0 m) and latitude (23°06' N—25°48' N),with space distribution along latitude; The reproductive characters were positively correlated to the annual mean temperature,annual rainfall (197.0-1 277.0m) and longitude (101 °51 '—103 °11' E),and negatively correlated to altitude and latitude,with space distribution along latitude and longitude.Euclid's genetic distance between populations ranged from 2.44 to 8.16.Mantel test showed that the genetic distance among populations was not significantly correlated to the geographic distance (R =0.28209,P =0.9463),difference of temperature (R =0.45666,P =0.9993) and difference of annual rainfall (R =0.233 26,P =0.9243),but was negatively correlate to difference of altitude (R =-0.417 03,P =0.003 2),suggesting that the altitude (with heterogeneous environment) might affect the genetic differentiation of populations.