采用正交试验设计,通过制作石蜡切片以及光学显微观察系统研究了植物生长调节剂对油茶芽苗砧嫁接愈合及嫁接苗成活的影响.结果表明:GGR6、IBA、芸苔素内酯能够明显促进愈伤组织的分化形成、砧木和穗条愈伤组织的连接与延伸,进而加快嫁接苗的愈合过程,各处理愈伤比例均值较对照的增幅为28.01％,且比对照至少提前15 d完成愈合;GGR6、IBA、芸苔素内酯的不同浓度及不同施用方法浸基质、蘸根、喷叶对愈伤比例的形成以及嫁接苗成活率的影响差异极显著.促进油茶芽苗砧嫁接愈合的最佳处理方式为:0.03 g·L-1GGR6、0.2 g·L-1IBA及0.14～0.28 mL·L-1芸苔素内酯混合液500 mL+浸基质1.42 L.
Effect of Plant Growth Regulators on the Healing of the Nurse Seed Grafted Unions of Camellia oleifera
In order to study the effect of plant growth regulators on the healing progress of the nurse seed grafted unions of Camellia oleifera,an orthogonal experiment of GGR6,IBA,Brassinolide,different concentration and application method (leaching medium,dunking root,and spraying leaves) on the nurse seed grafted seedlings of C.oleifera was carried out.The paraffin sections and microscopic observation system were used to measure the area of the callus and grafted union and get the healing rate,then the healing progress was made to be measurable.The results showed that the application of plant growth regulators significantly promoted the healing progress,the form of the callus and the connection of stock and scion.The average healing rate of different treatment was 28.01％ higher than that of the control group,and the time required for the grafted union to be healed was 15 days less than the control group.The effect of different concentrations and application methods of plant growth regulators on the healing progress and survival rate was significantly different,among these treatments,the best was 0.03 g · L-1GGR6,0.2 g · L-1IBA and 0.14 mL · L-1 ～ 0.28 mL · L-1 Brassinolide mixed liquid to leach medium 1.42 L per 500 mL.
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万方数据
维普
