Development of IRAP Markers Based on Genomic LTR Retrotransposon Sequences in Masson Pine (Pinus massoniana)
[Objective]To develop new molecular markers suitable for Pinus massoniana.[Method]Based on the conservative region of RT (reverse transcriptase)sequences of Ty1-copia and Ty3-gypsy type retrotransposon,the IRAP-PCR system for P.massoniana was established and examined with 12 individuals.[Result]Of 42 IRAP prim-ers,29 gave stable and polymorphic amplification profiles,thus yielded 227 bands,among which 207 were polymor-phic,accounting for 91.19% of the total.The average Observed Number of Alleles,Effective Number of Alleles, Nei’s Genetic Diversity and Shannon Index of Diversity in the twelve P.massoniana gemplasms were 1.911 9 ± 0.284 1,1.468 0 ±0.288 2,0.291 1 ±0.144 9 and 0.447 2 ±0.195 3,respectively.The cultivated types and clones could be effectively distinguished by primers P-12,P-15 or R-1 ,and all the genotypes might be clarified by P-2,which was labeled as the core primer for tested germplasms.The coefficient of the 12 germplasms ranged from 0.46 to 0.69,and with the threshold of 0.57,all the genotypes were grouped into three subclusters by the UPG-MA.[Conclusion]The IRAP marker technology established may effectively facilitate the identification of genetic relationship in P.massoniana germplasms.
NETL
NSTL
万方数据
维普
