Cloning and Yeast Expression Function Identification of High Expression XsKCS7 Gene in Xanthoceras sorbifolium Seed
[Objective]To explore the key genes of regulating nervonic acid biosynthesis in Xanthoceras sorbifolium seeds.[Method]This study analyzed the expression pattern of the 3-ketoesteryl-CoA syn-thase(3-ketoacyl-CoA synthase,KCS)gene family in seeds at different developmental periods according to the combined transcriptome of the reference genome.Gene XsKCS7 was cloned by RT-PCR,and was analyzed by Bioinformatics analysis of XsKCS7 protein.And the XsKCS7 gene was transformed into Sac-charomyces cerevisiae to identify the gene function.[Result]The expression level of XsKCS7 gene in seeds of X.sorbifolium was much higher than other KCS genes.The open reading frame of XsKCS7 was 1,512 bp,encoding 503 amino acids,havd three typical conserved domains of KCS family"GMGCSA","FGNTSSSS"and"GSGFKCNSAVW",and a close relationship with the homologous gene of Hevea brasi-liensis,at 67.62%.XsKCS7 heterologous transformation of Saccharomyces cerevisiae regulated the syn-thesis of erucic acid and nervonic acid.[Conclusion]:XsKCS7 gene is a key gene that regulates the syn-thesis of erucic acid and nervonic acid in seeds of X.sorbifolium.
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