Functional Conservation Existed in PagKNAT2/6a and PagKNAT2/6b During Xylem Development
[Objective]To analyze the regulatory mechanism of PagKNAT2/6a in poplar dendritic develop-ment and the functional conservation of PagKNAT2/6a and PagKNAT2/6b in xylem development.[Meth-ods]Gene sequence alignment analysis was carried out by using related software;Gene expression pat-tern analysis was performed by experiments such as GUS staining and real-time quantitative(qRT-PCR)of transgenic plants,and then stem anatomy analysis was also performed;Finally,genes related to sec-ondary wall synthesis(such as cellulose synthesis-related genes CESA4,CESA7A,CESA7B,etc.;Hemi-cellulose synthesis-related genes GUX1a,GUX1b,PARVUS;Expression analysis and regulatory mechan-ism of lignin synthesis-related genes COMT,CAD,LAC4,etc)were explored.[Results]The sequences of PagKNAT2/6a and PagKNAT2/6b were highly similar,and they were expressed in different tissues of'84K',showing higher expression levels in poplar stems,stem tips and young leaves.Stem anatomy ana-lysis of transgenic plants abnormally expressing PagKNAT2/6a showed that PagKNAT2/6a overexpres-sion inhibited xylem development compared with wild type.At the same time,qRT-PCR analysis showed that PagKNAT2/6a gene related the expression of genes related to secondary wall synthesis and affected xylem development.[Conclusion]PagKNAT2/6a and PagKNAT2/6b both negatively regulate xylem devel-opment and have functional conservation.
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