PagKNAT2/6a与PagKNAT2/6b在木质部发育中存在功能保守性
Functional Conservation Existed in PagKNAT2/6a and PagKNAT2/6b During Xylem Development
李海阳 1赵立子 1赵岩秋1
作者信息
- 1. 鲁东大学农林工程研究院,山东烟台, 264025;鲁东大学农学院,山东烟台, 264025
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摘要
[目的]通过比较分析PagKNAT2/6a与PagKNAT2/6b在木质部发育中的功能差异,解析KNOX基因在木材形成过程中的生物学功能.[方法]利用相关软件进行基因序列比对分析;通过对转基因植株进行GUS染色和实时荧光定量(qRT-PCR)等实验进行基因表达模式分析,随后对茎部解剖学分析,进而解析PagKNAT2/6a对木质部发育的影响;最后,对次生壁合成相关基因(如纤维素合成相关基因 CESA4、CESA7A、CESA7B等;半纤维素合成相关基因GUX1a、GUX1b、PARVUS;木质素合成相关基因COMT、CAD、LAC4等)的表达分析探究 PagKNAT2/6a对次生壁合成的调控机制.[结果]PagKNAT2/6a与PagKNAT2/6b序列高度相似,在'84K'不同组织中均有表达,在杨树茎、茎尖以及幼嫩叶片中表现出较高的表达量.对PagKNAT2/6a转基因植株的茎部解剖分析表明,与野生型相比,PagKNAT2/6a过表达抑制木质部发育;同时qRT-PCR分析表明PagKNAT2/6a基因通过调控次生壁合成相关基因的表达影响木质部发育.[结论]PagKNAT2/6a与PagKNAT2/6b均负调控木质部发育,同时存在功能保守性.
Abstract
[Objective]To analyze the regulatory mechanism of PagKNAT2/6a in poplar dendritic develop-ment and the functional conservation of PagKNAT2/6a and PagKNAT2/6b in xylem development.[Meth-ods]Gene sequence alignment analysis was carried out by using related software;Gene expression pat-tern analysis was performed by experiments such as GUS staining and real-time quantitative(qRT-PCR)of transgenic plants,and then stem anatomy analysis was also performed;Finally,genes related to sec-ondary wall synthesis(such as cellulose synthesis-related genes CESA4,CESA7A,CESA7B,etc.;Hemi-cellulose synthesis-related genes GUX1a,GUX1b,PARVUS;Expression analysis and regulatory mechan-ism of lignin synthesis-related genes COMT,CAD,LAC4,etc)were explored.[Results]The sequences of PagKNAT2/6a and PagKNAT2/6b were highly similar,and they were expressed in different tissues of'84K',showing higher expression levels in poplar stems,stem tips and young leaves.Stem anatomy ana-lysis of transgenic plants abnormally expressing PagKNAT2/6a showed that PagKNAT2/6a overexpres-sion inhibited xylem development compared with wild type.At the same time,qRT-PCR analysis showed that PagKNAT2/6a gene related the expression of genes related to secondary wall synthesis and affected xylem development.[Conclusion]PagKNAT2/6a and PagKNAT2/6b both negatively regulate xylem devel-opment and have functional conservation.
关键词
PagKNAT2/6a/PagKNAT2/6b/杨树/木质部发育/保守性Key words
PagKNAT2/6a/PagKNAT2/6b/Poplar/xylem development/Conservatism引用本文复制引用
基金项目
山东省高等学校青创团队计划(2022KJ120)
国家自然科学基金(32101482)
出版年
2024
国家科技期刊平台
NSTL
万方数据