日本落叶松胚状体干化处理对萌发的影响
Effect of Desiccation on Germination of Larix kaempferi Embryoids
孙海涛 1杨玲 2齐力旺 3李万峰3
作者信息
- 1. 林木遗传育种全国重点实验室东北林业大学林学院,黑龙江哈尔滨 150040;林木遗传育种全国重点实验室国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所,北京 100091
- 2. 林木遗传育种全国重点实验室东北林业大学林学院,黑龙江哈尔滨 150040
- 3. 林木遗传育种全国重点实验室国家林业和草原局林木培育重点实验室中国林业科学研究院林业研究所,北京 100091
- 折叠
摘要
[目的]提高日本落叶松胚状体的萌发能力,优化日本落叶松良种繁育技术.[方法]本研究根据子叶数量和胚轴形态,对胚状体进行分类,并分别统计其萌发率;利用"滤纸容器法"对类型Ⅰ胚状体进行干化处理,统计其萌发情况.[结果]胚状体分为 10种类型.类型Ⅰ胚状体萌发率为 4.48%,其余类型胚状体难以萌发.干化处理14d后,类型Ⅰ胚状体萌发率提高到69.43%,且具有干化响应的胚状体更易萌发.[结论]本研究表明日本落叶松胚状体干化处理后萌发率明显提高,为该物种良种繁育提供依据.
Abstract
[Objective]To improve the germination ability of Larix kaempferi embryoids and optimize the breeding technology of Larix kaempferi.[Method]In this study,we conducted classification on embryoids based on the number of cotyledon and the hypocotyl shape.Subsequently,their germination rates were re-corded respectively.The germination rate of typeⅠembryoids was evaluated after desiccation using"pa-per vessel method".[Result]Type Ⅰ embryoids exhibited an initial germination rate of 5%,while the re-maining types did not show any germination.However,after drying for two weeks,the germination rate of type Ⅰ embryoids significantly increased to 69.43%,and embryoids with desiccation response were more likely to germinate.[Conclusion]These results show that the germination rate of Larix kaempferi em-bryoids is significantly improved after desiccation,which provides insights into new strategies for improv-ing seedling production for this species.
关键词
日本落叶松/胚状体/萌发/干化/体细胞胚胎Key words
Larix kaempferi/Embryoid/Germination/Desiccation/Somatic embryo引用本文复制引用
基金项目
国家自然科学基金面上项目(32271904)
出版年
2024
国家科技期刊平台
NSTL
万方数据