Effect of Endophytic Bacteria on Seedling Growth of Falcataria falcata
[Objective]To reveal the composition of dominant nodule endophytes in Falcataria falcata and explore their effect on the growth of Falcataria falcata seedlings,thus providing information for the develop-ment of Falcataria falcata fertilizer.[Methods]Endophytic bacteria were screened from Falcataria falcata nodules collected from three locations(Guangzhou,Huizhou and Maoming of Guangdong province),and inoculation with the three different geographical nodule endophytes(YZ,YD and YX)were conducted.The growth traits of Falcataria falcata seedlings after inoculation with different geographical endophytic bac-teria were measured.Furthermore,species identification of endophytic bacteria with different treatments was performed by using 16S rRNA gene sequencing technology.[Results]Compared with the CK(negat-ive control),Falcataria falcata seedlings under the three treatments were able to develop nodules success-fully,and the seedling growth and nodule number,biomass,photosynthesis and nitrogen fixation ability were significantly higher than those under the control treatment.Particularly,the seedling height,root length,SPAD in the three treatments increased by 53.74%～95.65%,52.91%～98.41%,74.04%～86.18%,and 7.6～15.9 times compared to the control,respectively.Among the three inoculation treatments,seed-ling after inoculation with YZ showed the highest values of seedling growth,nodulation number and nitro-gen fixation.Significant differences in the composition of endophytic bacteria were found among the three treatments,and YZ treatment had the largest number of OTU number and the highest diversity of α(Shannon index).The denitrifying Fe-Ⅱ oxidizing bacterium had significant effects on seedlings height,nod-ules number,nitrogen concentration,nitrogen content and nitrogen fixation in Falcataria falcata.[Conclusion]The endophytes isolated from Falcataria falcata nodule can promote seedling growth,bio-mass accumulation,photosynthesis ability and nitrogen fixation in Falcataria falcata seedlings.Different geographical nodule endophytes show different composition of endophytic bacteria and varying effects on seedling growth.Endophytes with high diversity have obvious growth-promoting effect on Falcataria falc-ata seedlings.
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万方数据
