Selection of Reference Genes for Gene Expression Analysis in Seed Development of Xanthoceras sorbifolium Bunge
[Objective]:To assess the suitability of the ACT gene for gene expression analysis in seed de-velopment of Xanthoceras sorbifolium,and to identify the most stable reference genes for gene expres-sion analysis in X.sorbifolium.[Method]:In this study,four mathematical algorithms(ΔCT,BestKeeper,NormFinder,geNorm)were carried out for evaluating the expression stability of 12 candidate reference genes based on the web-based tool RefFinder.[Result]:The RefFinder result showed that the gene PP2A performed the best,followed byTip41,EF-1α,18r,UCE,β-TUB,UBQ,α-TUB,CYP,SAM,ACT,and GADPH at the different seed development stages in X.sorbifolium,and the gene also had the highest score,followed by Tip41,CYP,18r,UBQ,SAM,EF-1α,UCE,GADPH,α-TUB,ACT,and β-TUB at various tissues.[Conclusion]:PP2A and Tip41 are the two most stable reference genes for gene expression ana-lysis in X.sorbifolium.
Xanthoceras sorbifoliumreference genegene expressionseed development
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