叶面喷施硒肥对香榧幼苗叶片代谢组的影响
Effects of Foliar Spray of Selenite on Metabolome in Leaves of Torreya grandis Seedlings
陈振生 1李爱华 2杜拾平 2黄培耕 1许秀环 2宁博林 1李文1
作者信息
- 1. 广西国有博白林场,广西博白 537617
- 2. 湖北省林业科学研究院,湖北武汉 430075
- 折叠
摘要
[目的]分析亚硒酸钠叶面喷施后对香榧(Torreya grandis)幼苗叶片代谢组的影响,探究亚硒酸钠喷施后香榧叶片次生代谢物的累积情况及其对果实品质的影响.[方法]以苗龄2 年的香榧幼苗为供试材料,叶面喷施亚硒酸钠(浓度为100 μg·L-1)2次,间隔15 d.第二次喷施后15 d,采取1年生叶片进行代谢组分析,以色谱-质谱研究香榧幼苗叶片代谢物差异.[结果]在正离子模式下,处理组和对照组之间有909种差异代谢物,其中 69种显著变化(27种显著上调,42种显著下调).在负离子模式下,出现 433种差异代谢物,其中 43种显著变化(11种显著上调,32种显著下调).对人体有益的一些次生代谢物显著增加,如车前苷(2.827倍)、川芎内酯(2.524倍)、6-唾液乳糖(2.021倍)、阿齐沙坦(1.931倍).[结论]香榧叶片喷施亚硒酸钠有利于促进对人体有益的次生代谢物的生物合成及其在叶片中的累积.
Abstract
[Objective]To investigate the effects of foliar spray of selenite on metabolome in the leaves of Torrey grandis seedlings and changes in levels of secondary metabolites in the leaves.[Method]Sodium selenite of 100 μg·L-1 was sprayed to the leaves of 2-year-old seedlings of T.grandis at an interval of 15 days.15 days after the second spraying,one-year-old leaves were harvested for metabolome analysis.[Result]The results showed that there were 909 differential metabolites between treatment group and control under positive ionization mode,with 69 metabolites of significant changes(27 metabolites up-regu-lated and 42 metabolites down-regulated).Under negative ionization mode,433 differential metabolite oc-curred between treatment group and control,with 43 differential metabolites of significant changes(11 metabolites up-regulated and 32 metabolites down-regulated).Some metabolites beneficial to human health increased significantly in the treated leaves of T.grandis seedlings,such as plantagoside(increas-ing 2.827 times as control),ligustilide(increasing 2.524 times),6-sialyllactose(increasing 2.021 times),and azilsartan(increasing 1.931 times).[Conclusion]The results indicate that selenite foliar application on the leaves of T.grandis can increase biosynthesis of beneficial metabolites and accumulation in their leaves.
关键词
香榧/硒肥/代谢组/生物强化Key words
Torreya grandis/selenium fertilizer/metabolome/biofortification引用本文复制引用
基金项目
湖北省林业科技支撑计划重点项目(2016-LYKJ01)
出版年
2024
国家科技期刊平台
NSTL
万方数据