Analysis of Transcription Factor ZF-HD Gene Family and Expression Pattern of Eucalyptus grandis
[Objective]In order to analyze the role of the transcription factor ZF-HD in the growth and devel-opment in Eucalyptus and to further understand its function,ZF-HD gene family in eucalyptus.[Method]In this study,bioinformatics was used to identify members of the E.grandis ZF-HD gene family,and their chromosomal location,physical and chemical properties,gene structure,protein conserved domains and expression patterns were analyzed.[Result]A total of 10 ZF-HD gene members were identified in E.grandis,which encoded 78-343 amino acids.Meanwhile,almost all genes were basic proteins which mainly located in the nucleus.Moreover,motif1 is very conserved in the ZF-HD family,and their cis-acting promoter elements mainly included hormone response,growth and development,and stress response ele-ments.Furthermore,the expression analysis of ZF-HD gene in various tissues,adventitious roots and buds inducing process was analyzed.The results showed that most of the family members were differen-tially expressed in various tissues,which mainly acted in the downward direction of stem apex,Meanwhile,the expression levels of ZF-HD family members altered significantly during adventitious root and bud in-duction.EgrZHD2 expressed in higher level at the stem apex and decreased significantly with gradually in-creased secondary growth.And the further subcellular localization analysis showed EgrZHD2 localized in cell nucleus,which was consistent with predicted results.[Conclusion]The ZF-HD gene family members of Eucalyptus grandis play an important regulatory role in the development of adventitious roots and ad-ventitious buds.EgrZHD2 acts as an important transcription factor in the biological processes of primary growth and adventitious root induction in Eucalyptus.
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