Screening,Identification and Biological Characteristics of Biocontrol Fungi against Larch Shoot Blight
[Objective]The aim of the experiment is to obtain the biological control strain of larch shoot blight.[Method]Strains with strong inhibitory effect on Neofusicoccum laricinum was screened through plate antagonism screening experiments and re-screening with fermentation broth.The screened strains were identified and their biological characteristics were studied.[Result]A soil fungi T2 was screened that had a significant antagonistic effect on Neofusicoccum laricinum,which was identified as Trichoderma hamatum by morphology and molecular biology.Its Inhibition rate of plate experiments reached 74.16%,and fermentation broth experiments reached 91.29%.The fermentation broth had strong inhibition effect on a variety of plant pathogenic fungi,and showed strong inhibition effect on Sphaeropsis sapinea,Cyto-spora chrysosperma,Phyllosticta populea and Rhizoctonia solani with 92.00%inhibition rate and had bet-ter inhibition effect on Fusarium sporotrichioides and Marssonina brunnea with 79.67%and 62.67%inhibi-tion rate,too.T2 can utilize a variety of carbon and nitrogen sources,has a wide adaptability to PH and a high lethal temperature.[Conclusion]The antagonistic effect of T2(Trichoderma hamatum)on Neofusi-coccum laricinum is significant,with a wide spectrum of pathogenic inhibition and strong environmental ad-aptability.This study can provide theoretical research and foundation for the control of larch shoot blight.
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