Determination of the Control Effect of 2%Emamectin Benzoate Emulsifiable Concentrate on Pine Wood Nematode Disease
[Target]To explore the control effect of 2%emamectin benzoate emulsifiable concentrate(trade name:'Songgaofei')on pine wilt disease,[Methods]this study first compared the nematicidal activity of 2%emamectin benzoate emulsifiable concentrate and 2%emamectin benzoate microemulsion(trade name:'Linyuan Songyun')through the indoor immersion method;Then,the control effect of'Songgaofei'on pine wilt disease was tested through trunk injection in the greenhouses and in the forest fields;Finally,the content of emamectin benzoate(abv.EB)in pine trees at different time points was determined by high-performance liquid chromatography(HPLC).[Results]The results of the indoor immersion method showed that'Songgaofei'had stronger nematicidal activity,with an LC50 of 41.808 mg/L,which was significantly lower than the 75.959 mg/L of'Linyuan Songyun'(p<0.05).In the prevention group,'Songgaofei'dis-played good protection results with no pine death of nemantode after 2 years;However,in the treatment group,the earlier the treatment was conducted,the better the treatment effect was;all the pine trees did not die when injection'Songgaofei'7 days after inoculation of nemantode,while in the group injected'Songgaofei'30 days after inoculation,P.tabuliformis had a mortality rate of 20%,and P.armandii had a mortality rate of 40%,other than no death of Pinus bungeana.Field experiments in two sites showed that the control effects of'Songgaofei'were significant,with the 2-year cumulative mortality rates of P.masso-niana in the experimental group being 2%and 5%,much lower than the control groups mortality rates of 20%and 25%,respectively.Residue levels of'Songgaofei'in different pine species changed with time,the average content(36.59 mg·kg-1)in P.armandii was the highest,and the P.tabuliformis(27.11 mg·kg-1)is the lowest.Within15 days after injection,the residue of'Songgaofei'in all pine species increased.After 15 days,the residue of'Songgaofei'in all parts of tree trunk gradually decreased and stabilized fi-nally in both P.bungeana and P.tabuliformis,except the central trunk of P.armandii,which continuously increased until day 60.[Conclusion]The 2%emamectin benzoate emulsifiable concentrate'Songgaofei'exhibits significant nematicidal activity against pine wood nematodes,with remarkable control effects in both greenhouse and field conditions.'Songgaofei'is well-transmitted and well remained within different pine species without causing noticeable phytotoxicity,indicating a potential application in practice.
Pine wood nematode diseaseEmamectin benzoate emulsifiableNematicidal activityPrevention and control effectResidues
万方数据
维普
