[Objective]The aims of the study are to screen the main volatile components in the experiment-al forests of poplar-A.asphodeloides,poplar-C.morifolium,and poplar-M.canadensis compound manage-ment,and determin their repellent or attractant effects on Anoplophora glabripennis and Hyphantria cunea indoors.[Method]The dynamic headspace adsorption method was used to collect volatile compounds from the aboveground,the whole plants of three medicinal plants,and the air in the poplar-herb compound management experimental forests.GC-MS was used to analyze and identify the volatile components,and screened the main volatile compounds.The EAG and olfactory behavioral responses of A.glabripennis and H.cunea were tested in the laboratory.[Result]The main volatile compounds,cis-3-Hexenyl acetate,beta-Caryophyllene and menthol,were screened from experimental forests of poplar-A.asphodeloides,poplar-C.morifolium,and poplar-M.canadensis,respectively.All three compounds could induce EAG re-sponses in the tested concentration range,and the values increased with the raise of concentration.Menthol had a significant repellent effect on the adults or larvae of A.glabripennis and H.cunea,with the highest repellent rates of 65％and 47.5％on female and male A.glabripennis at 60 μg·μL-1 concentration,while the highest repellent rates of 60％and 70％on adult and larval H.cunea at 500 μg·μL-1 concentra-tion,respectively.Beta-Caryophyllene could attract male insects of two species,with the attractive rate ranging from 40％to 47.5％.Different concentrations of cis-3-Hexenyl acetate induced differentiation ef-fects on female and male A.glabripennis and adult H.cunea,with a low concentration attraction rate of about 45％,but a high concentration repellent rate of about 50％.While,it had a repellent rate of 52.5％on female adults of H.cunea and an attractive rate of 45％～65％on H.cunea larvae.[Conclusion]Cis-3-Hexenyl acetate,β-Caryophyllene and Menthol,the main volatile components in the 3 experimental forests,can both induce EAG and olfactory behavioral responses in A.glabripennis and H.cunea.Menthol has the most obvious effect with a significant repellent effect on two insects And the following is Cis-3-Hex-enyl acetate,mainly manifested as low concentration attraction and high concentration repellent effect.While,β-Caryophyllene has the worst effect with an attractive effect on two species of male insects only.These results can provide a basic theoretical basis for the development of plant-based attractants and re-pellents,as well as the construction of a green and environmentally friendly poplar compound manage-ment model based on the"push-pull"control strategy.
关键词
杨药复合经营/光肩星天牛/美国白蛾/挥发物/GC-MS/EAG/行为反应
Key words
the poplar-herb compound management/Anoplophora glabripennis/Hyphantria cunea/volatiles/GC-MS/EAG/behavioral responses
维普
万方数据