Genetic Differentiation and Phylogeographic Analysis of Neuroctenus yunnanensis Based on Mitochondrial Genome
[Objective]The aims of the study are to analyze the genetic diversity and phylogeny relation-ships of Neuroctenus yunnanensis in Yunnan Province of China,to speculate its origin and divergence time,and to explore the causes of its historical biogeographic distribution pattern.[Method]The mitochon-drial genome of N.yunnanensis collected from 10 regions in Southwest China from 2009 to 2020 was se-quenced using high-throughput sequencing.Genetic diversity was analyzed by MEGA11,DnaSP6,and Ar-lequin 3.5.2.2 software.The phylogenetic tree of N.yunnanensis was reconstructed using PhyloSuite v1.2.3,and eight other species from the Aradidae were used as outgroups.The divergence time of N.yun-nanensis was estimated using BEAST v1.10.4 software,incorporating loose molecular clock and fossil cor-rection points.[Result]In this study,30 mitochondrial protein-coding sequences with a length of 108 90 bp were obtained.A total of 26 haplotypes were detected,with a haplotype diversity Hd of 0.989.The nucle-otide polymorphism index π was 0.026 21,showing high Hd and low π.AMOVA analysis suggested that the genetic differentiation in N.yunnanensis mainly occurred among populations.Phylogenetic analysis showed that the population of N.yunnanensis in Southwest China was obviously divided into four clades,and the haplotype network diagram was consistent with the results of the phylogenetic tree.Divergence time estimation indicated that N.yunnanensis began to differentiate in the middle Miocene of 13.83 Ma.The Lvchun population(in the south of the Ailao Mountains)was the original population in China and then divided into three clades along the Hengduan Mountains.[Conclusion]The Quaternary glacial-interglacial cycle event has formed the current geographical differentiation pattern of N.yunnanensis in Southwest China.
万方数据
维普
