Genetic Differences of Rosa roxburghii Tratt.Using SSR Primers from Different Sources
[Objective]The objective of this was to study the differences in the genetic analysis of R.roxburghii population between the EST-SSR of R.roxburghii and its related species Rosa chinensis,in or-der to provide SSR markers from different sources for the study of R.roxburghii genetic diversity and struc-ture.[Method]Six pairs of R.chinensis EST-SSR primers and six pairs of R.roxburghii EST-SSR primers were used to genotype 261 wild R.roxburghii samples.GenAlex software was used to estimate population genetic diversity parameters,genetic differentiation coefficients,and gene flow,and AMOVA molecular variance analysis was performed.Variance analysis and correlation analysis were performed using SPSS software,and genetic similarity and cluster were analyzed according to UPGMA method through NTSYS software.[Result]A total of 95 bands were amplified by 12 pairs of SSR primers,of which 53 bands were amplified by R.chinensis SSR primers and 42 bands were amplified by R.roxburghii SSR primers.The av-erage expected number of alleles,expected heterozygosity,polymorphism information content,and Shan-non's information index estimated based on Rosa chinensis Jacq.SSR primers were 2.206,0.477,0.459,and 0.862,respectively.They were 1.242,0.205,0.215,and 0.451 lower than the corresponding indicat-ors estimated based on Rosa roxburghii Tratt.SSR primers,respectively.The genetic differentiation res-ults showed that the estimated genetic differentiation coefficients(FST)based on rose SSR and R.roxburghii SSR were 0.128 and 0.062,respectively,indicating that the population belongs to a lower level of genetic differentiation.The results of population AMOVA analysis based on two SSR markers were very similar,and genetic variation mainly came from within the population.The genetic distance correlation results showed that the results obtained from both sources of SSR were significantly correlated with the results obtained from mixed SSR,and the clustering results were also basically consistent.[Conclusion]There are certain differences in genetic diversity and distance analysis between R.chinensis Jacq.SSR and R.roxburghii Tratt.SSR,but the differences are not significant,indicating that the SSR of closely related species has good universality in R.roxburghi.It provides markers for subsequent collection of R.roxburghii germplasm resources,variety identification,and fingerprint construction,and provide new ideas for the analysis of Rosaceae plant genetic diversity.
Rosa roxburghii Tratt.Rosa chinensis Jacq.EST-SSRgenetic diversitycorrelation analysis
万方数据
维普
