Effects of Seed Soaking and Sowing Temperatures Increasing on Seed Germination and Related Inclusion and Enzyme Activity of Cyclobalanopsis gilva
[Objective]To explore the physiological mechanisms underlying the effects of soaking and in-creased sowing temperature on the germination characteristics,related inclusion content,and enzyme activity changes of Cyclobalanopsis gilva seeds,providing a scientific basis for improving their germination rate and uniformity.[Method]Based on the mature seeds of C.gilva,a factorial design experiment was conducted involving two factors:two sowing temperature treatment and four soaking treatment.Seed ger-mination was regularly monitored after sowing,and randomly selected seeds were used to measure inclu-sion content and enzyme activity.[Result]Increasing sowing temperature at various soaking levels ad-vanced the initial germination time of C.gilva seeds.Long-term seed soaking and elevated sowing temper-atures effectively improved the average germination speed,reduced the average germination time and in-creased the germination rate of seeds.Changes in inclusion content and enzymatic activity varied signific-antly during seed germination,with consistent patterns observed across different sowing temperatures.Starch content initially increased before decreasing,while soluble sugars and soluble proteins decreased before increasing.Amino acid content and the activities of CAT,POD,and PPO exhibited fluctuating down-ward trends,except for SOD,which remained stable.The separate seed soaking treatment did not signific-antly affect changes in inclusion contents and enzyme activity during germination.However,increased sowing temperature advanced the peak time of POD activity,with longer seed soaking times resulting in earlier peak times.This combination also accelerated starch hydrolysis,reduced fluctuations in amino acid content,and increased consumption,facilitating faster seed dormancy breaking and germination.In April,higher soluble sugar content was associated with poorer germination uniformity;higher amino acid content and PPO activity correlated with earlier initial germination and shorter germination durations;and higher PPO activity was linked to greater germination potential.[Conclusion]Long-term seed soaking and in-creasing sowing temperatures can effectively accelerate germination speed,improve germination rate,and enhance uniformity.Under the conditions of increased sowing temperature on a seedbed with a small arched shed in a steel greenhouse,C.gilva seeds soaked for 48 hours has the earliest initial germination time(82 days),the highest germination rate(71.00%),the highest germination index,and the strongest germination ability.
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