Screening of Excellent Fungi-tree Combinations for Phosphorus Absorption Promotion of Pinus massoniana
[Objective]This paper aimed to screen the excellent fungi-tree combinations of Pinus massoniana that significantly promote phosphorus absorption.[Method]2-month-old potted Pinus massoniana seedlings were selected as experimental subjects,and ectomycorrhizal fungi were inoculated to form ectomycorrhizal fungi.The strains were selected as follows:Pisolithus tinctor(P.t),Suillus bovinus(S.b),Rhizopogon luteolus(R.l),Suillus luteus(S.l).The single inoculation and the mixed strains of 4 strains were inoculated respectively.At the same time,one inoculant inactivated mixed strains was set as the control,and the seedling substrate was pure vermiculite.After 3 months of inoculation,the seedlings that successfully formed ectomycorrhiza were treated with three different phosphorus concentrations,respectively set as P1(2 mg·L-1),P2(6 mg·L-1)and P3(10 mg·L-1).After two months of culture,the mycorrhiza infection rate,seedling growth,physiological and biochemical indexes of different fungi-tree were detected,so as to screen excellent P.massonii tree combinations that promote phosphorus absorption.[Result]Under P1 condition,the mycorrhiza infection rate,seedling height,biomass and MDA content of Pinus massoniana seedlings treated with S.l were superior to those treated with other treatments.The chlorophyll and proline contents of Pinus massoniana seedlings under R.l treatment were the best.The total P content of Pinus massoniana seedlings under S.b treatment was the highest.Under P2 condition,the seedling height,total P content and MDA content of Pinus massoniana seedlings under S.l treatment were better than those under other inoculation treatments.The mycorrhiza infection rate,chlorophyll content and proline content of Pinus massoniana seedlings under R.l treatment were the best.The biomass of Pinus massoniana seedlings under S.b treatment was better than that under other inoculation treatments.Under P3 condition,the mycorrhizal infection rate,biomass,chlorophyll and total P of Pinus massoniana seedlings treated with R.l were better than those treated with other inoculation treatments.The seedling height,proline and MDA contents of Pinus massoniana seedlings under S.l treatment were the best.[Conclusion]The inoculation of S.l significantly improved the phosphorus absorption energy of Pinus massoniana in the phosphorus-deficient environment,and the optimal fungi-tree combination is S.l with Pinus massoniana symbiosis.
万方数据
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