温带林业研究2024,Vol.7Issue(3) :17-21.DOI:10.3969/j.issn.2096-4900.2024.03.003

黑龙江省山杨倍性调查分析

Survey and Analysis of Populus davidiana Ploidy in Heilongjiang Province

王宇函 王鑫宇 徐祥宇 路美琪 梁雪莹 陈肃 陈安琪
温带林业研究2024,Vol.7Issue(3) :17-21.DOI:10.3969/j.issn.2096-4900.2024.03.003

黑龙江省山杨倍性调查分析

Survey and Analysis of Populus davidiana Ploidy in Heilongjiang Province

王宇函 1王鑫宇 1徐祥宇 1路美琪 1梁雪莹 2陈肃 1陈安琪2
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作者信息

  • 1. 东北林业大学林木遗传育种全国重点实验室,黑龙江哈尔滨 150000
  • 2. 黑河市中俄林业科技合作园区,黑龙江黑河 164300
  • 折叠

摘要

[目的]多倍体具有器官巨大性和抗逆性强等特点,筛选多倍体一直是获得林木优良品种的方法之一.山杨生长速度快,是土壤改良、防风固沙工程上的重要树种.因此,调查不同分布区的山杨倍性,对筛选优良杨树多倍体具有指导意义.[方法]使用倍性分析仪和荧光原位杂交技术(FISH)对大兴安岭、伊春和牡丹江地区的山杨进行倍性分析.[结果]大兴安岭地区的 31 个山杨样品中存在 6 个三倍体植株.而伊春和牡丹江地区 14 和 44 个山杨样品均为二倍体植株.[结论]在大兴安岭地区存在天然的三倍体山杨.

Abstract

[Objective]Polyploidy is characterized by organ gigantism and strong resistance,and screening polyploidy has been one of the methods to obtain excellent varieties of forest trees.Populus davidiana has a fast growth rate and is an important species on soil improvement and windbreak and sand fixation control projects.Therefore,investigating the ploidy of Populus davidiana in different distribution areas is the guiding significance for screening excellent poplar polyploids.[Method]Ploidy analysis of poplars from Daxing'an Mountains,Yichun city and Mudanjiang city were carried out using the PA and fluorescence in situ hybridization(FISH).[Result]Six triploid plants were present in 31 poplar samples from Daxing'an Mountains.In contrast,14 and 44 poplar samples from Yichun city and Mudanjiang city were diploid plants.[Conclusion]Natural triploid Populus davidiana exists in Daxing'an Mountains.

关键词

山杨/三倍体/荧光原位杂交/倍性分析

Key words

Populus davidiana/triploid/fluorescence in situ hybridization/ploidy analysis

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基金项目

东北林业大学大学生创新项目(DC-2024224)

出版年

2024
温带林业研究
国家林业局哈尔滨林业机械研究所

温带林业研究

影响因子:0.198
ISSN:2096-4900
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