Review of Single-cell RNA Sequencing and Chromatin Accessibility Sequencing Technologies in Plants
Cellular heterogeneity,a ubiquitous characteristic of biological tissues,poses challenges in elucidating genomic,transcriptomic,and proteomic variations that traditional sequencing methods struggle to address.The emergence of single-cell sequencing technologies has revolutionized our ability to unravel these complexities.Specifically,non-coding chromatin dynamics play a pivotal role in gene transcription regulation,and chromatin accessibility sequencing technologies,such as ATAC-seq(Assay for Transposase-accessible Chromatin using sequencing),offer researchers genome-wide insights into open chromatin regions.Initially applied to animal and human tissue studies,single-cell RNA sequencing(scRNA-seq)and ATAC-seq have,in the past five years,gained traction in plant research.High-throughput sequencing at the single-cell level has emerged as a powerful means to analyze plant cellular heterogeneity landscapes.Chromatin accessibility sequencing,in particular,is a crucial technique for elucidating regions of chromatin that are open or closed.In recent years,there has been a marked increase in the publication of studies utilizing these technologies in plant research.This paper delved into the principles and methodologies of these novel technologies,their applications,and their documented contributions to plant science research,and aimed to provide a comprehensive overview of scRNA-seq,scATAC-seq,and their integration in elucidating the intricate mechanisms underpinning plant cellular heterogeneity and gene regulation.
single-cell RNA sequencingassay for transposase-accessible chromatin using sequencingcellular heterogeneity
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