云恢 290 是云南高原特色籼型稻谷品种之一，由于其独特的风味广受消费者喜爱。采用广泛靶向代谢组学方法对3个不同产地的云恢 290 稻谷进行代谢组测定，共鉴定出1 003种代谢物。通过主成分分析(PCA)、偏最小二乘法判别分析(OPLS-DA)，结果表明，在不同比较组间(GJYH290 vs MLYH290，MZYH290 vs GJYH290，MZYH290 vs MLYH290)的差异代谢物分别为 130、181、192 种，其中只有6 种差异代谢物均存在于3个不同比较组中，主要的代谢通路是氨基酸代谢和黄酮类物质代谢。3 个不同比较组中差异倍数最大的前 10种上调和下调代谢物主要是黄酮类物质、酚酸类物质、核苷酸及其衍生物等。本研究为探索不同种植地稻谷的代谢组成和差异等具有参考作用。
Study on Effect of Producing Region on the Metabolome Composition of Rice
Yunhui 290 is one of the characteristic indica rice varieties in Yunnan Plateau.Due to its unique flavor,Yunhui 290 is widely loved by consumers.In this study,the Yunhui 290 rice metabolome from 3 different producing areas was andyzed using a widely targeted metabolomics approach,and 1003 metabolites were identified.By principal component analysis(PCA)and partial least square discriminant analysis(OPLS-DA),the results showed that the numbers of differential metabolites between the comparison groups(GJYH290 vs MLYH290,MZYH290 vs GJYH290,MZYH290 vs MLYH290)were 130,180,and 192,respectively,only 6 different metabolites were common to all three comparison groups,with the main metabolic pathways involving amino acid metabolism and flavonoid metabolism.The top 10 most significantly upregulated and downregulated metabolites with the largest fold changes in the 3 comparison groups were mainly flavonoids,phenolic acids,nucleotides and their derivatives.This study provides valuable insights into the metabolic composition and differences of rice from different producing regions.
万方数据
维普
