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醇法加工花生蛋白功能特性及组成研究

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不同醇法条件制备的花生蛋白品质不同,导致蛋白性质出现差异,产品的功能特性与用途发生改变。以花生粕为原料,采用响应面法以产品得率为指标,优化条件为:体积分数75。00%、浸提温度35。00℃、浸提时间55。45 min,该条件下产品得率87。82%,蛋白含量58。02%(D1蛋白)。以蛋白含量为指标,优化条件为:体积分数67。12%、浸提温度42。02℃、浸提时间58。28 min,此时产品得率86。08%,蛋白含量61。10%(D2蛋白)。与对照组花生粕(CP)相比,D1、D2的蛋白含量分别提高5。72%、8。80%,乳化性、起泡性、持水性、持油性及凝胶性提高;D1、D2蛋白产品粒径减小,溶于水溶液中的清蛋白、伴花生球蛋白Ⅰ含量增加,表面疏水性增加,界面张力下降,花生蛋白的功能特性改变。
Effect of Ethanol Processing on Functional Properties and Composition of Peanut Proteins
The quality of peanut protein prepared under different ethanol processing conditions varies,leading to differences in protein properties and subsequently in the functional characteristics and applications of the protein products.Response surface methodology was employed to optimize the preparation conditions and analyze the functional properties of the peanut proteins.The protein optimized for product yield,designated as D1,was obtained under the conditions of 75.00%volume fraction,35.00℃extraction temperature,and 55.45 min extraction time,resulting in a product yield of 87.82%and a protein content of 58.02%.The protein optimized for protein content,designated as D2,was prepared with a 67.12%volume fraction,42.02℃extraction temperature,and 58.28 min extraction time,achieving a protein content of 61.10%and a product yield of 86.08%.Compared with control peanut(CP),the protein content of D1 and D2 increased by 5.72%and 8.80%respectively.Improvements were observed in foaming capacity,emulsifying properties,water and oil retention,and gel-forming abilities.The particle size of D1 and D2 protein products was reduced,while the contents of albumin and conarachin dissolved in water increased.Additionally,surface hydrophobicity increased,and interfacial tension decreased,leading to significant improvements in the functional properties of peanut proteins.

peanut proteinethanol extractionresponse surfacefunctional characteristicscomponents

张相风、郭兴凤、张倩、朱婷伟、赵树超、张明、林凤岩

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河南工业大学 粮油食品学院,河南 郑州 450000

山东凯斯达机械制造有限公司,山东 济宁 272000

济宁市机械设计研究院,山东 济宁 272000

花生蛋白 醇法提取 响应面法 功能特性 组分

2025

粮油食品科技
国家粮食局科学研究院

粮油食品科技

北大核心
影响因子:0.684
ISSN:1007-7561
年,卷(期):2025.33(1)