Transcriptome Analysis of Cryptolestes ferrugineus under Low-temperature Stress
Cryptolestes ferrugineus,a cosmopolitan stored-grain pest,was studied using transcriptome sequencing to investigate its molecular response to low-temperature stress..Combining PacBio Iso Sequencing and Illumina RNA Sequencing,10,508 full-length transcripts were obtained,with 9,523 successfully annotated.Compared to the control group,477 genes exhibited significant differential expressed under low-temperature stress,including 177 upregulated and 300 downregulated genes.KEGG analysis revealed that differentially expressed genes were primarily enriched in metabolic pathways,fatty acid biosynthesis,and secondary metabolite biosynthesis.RT-qPCR was used to analyze the expression levels of six differentially expressed genes:heat shock protein,fatty acid synthase,chitinase,trehalose transporter,cytochrome P450,and vitellogenin..The results demonstrated consistency between RT-qPCR and transcriptome sequencing analysis.This study offers preliminary insights into the molecular mechanisms of C.ferrugineus adaptation to low-temperature stress,forming a foundation for applying low-temperature grain storage technology in integrated pest management strategies.
Cryptolestes ferrugineustranscriptome analysislow temperature stressdifferentially expressed genesRT-qPCR
万方数据
维普
