Process Optimization of Simultaneous Saccharification and Fermentation for Cellulosic Ethanol Production from Kitchen Waste
In order to improve the cellulosic ethanol yield,the process conditions of cellulosic ethanol production by simultaneous saccharification and fermentation(SSF)of kitichen waste were optimized by using undecayed fruit peels and vegetable wastes as the raw materials,and brewer's yeast and cellulase enzyme are adopted as the catalytic additives in a single-factor experiment and orthogonal experiments.The results showed that,in the one-way experi-ment,the cellulase enzyme dosage,yeast dosage,fermentation temperature and initial pH could increase the ethanol produc-tion rate when SSF process was used,and the differences were not significant;In orthogonal experiments,when using the SSF process,the factors affecting ethanol yield from strongest to weakest are fermentation temperature>cellulase dosage>yeast dosage.Under the conditions of cellulase dosage of 30 mg,yeast dosage of 10 mL,initial pH of 5,fermentation temperature of 45℃,and fermentation for 72 hours,the highest ethanol concentration reaches 6.43 g/L,with a yield of 0.089 g/(L·h).Research has found that adopting SSF technology provides a new direction for improving ethanol yield,achieving the resource utilization of waste,and alleviating the pressure of energy shortage.
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