The study of efficient degradation of N,N-dimethylformamide(DMF)using microorganisms is of great challenge.Screening and obtaining efficient DMF-degrading bacterial flora through anoxic acclimatization(HA)is the key to study DMF wastewater being biochemically degraded.The results of microbial community amplicon se-quencing(16S rDNA)showed that Proteobacteria,Firmicutes,and Euryarchaeota were the dominant phyla for de-grading DMF in the domesticated hypoxic bacterial community.Among them,Zavarzinia,Petrimonas,Acholeplas-ma,Brevundimonas,Arenimonas,and Bosea were able to effectively degrade DMF under hypoxic conditions,and the relative abundance of DMF degrading bacteria was significantly correlated with DMF degradation efficiency.When the relative abundance of DMF-degrading bacteriain HA reached 86.37％,the removal efficiency of DMF by HA sludge reached 99.98％for 210 mg·L-1.The products of DMF degradation by DMF-degrading bacteriawere dime-thylamine(DMA)and methylamine(MMA).The removal efficiency of DMF by HA sludge was only 57.22％when the relative abundance of DMF-degrading bacteriawas lower than 40.25％,and DMA showed a significant inhibitory effect on DMF-degrading bacteria.In this study,an anoxic-oxic(AO)processwas used to further improve the effluent water quality,and the concentrations of DMA and MMA in the aerobic sludge(AS)effluent were less than 0.5 mg·L-1,and the chemical oxygen demand(COD)in the effluent from the AS reactor was ≤40.6 mg·L-1,which meets the emission standard of pollutants for synthetic leather and artificial leather industry GB 21902-2008.
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