In order to improve the fixation rate of reactive dyes,response surface methodology was used to optimize the dyeing process of cotton fabric in D5 system in non-aqueous and less water medium.The interaction among sodium carbonate concentration,fixation time and fixation temperature was studied,and the optimum process parameters were optimized:sodium carbonate concentration 48 g/L,fixation time 10min and fixation temperature 89℃.According to the optimum process conditions,the predicted fixation rate is 75.67%.The fixation rate calculated by experiment is 76.73%,which is consistent with the predicted value.