高效液相色谱串联质谱法测定天然染料中的羟基红花黄色素A
Determination of hydroxy safflower yellow A in natural dyes by high performance liquid chromatography-tandem mass spectrometry
朱兰芳 1高慧红 1丁力进1
作者信息
- 1. 苏州市纤维检验院,江苏 苏州 215500
- 折叠
摘要
为鉴别天然染料中羟基红花黄色素A区别于化学合成染料,采用高效液相色谱串联质谱法建立一种天然染料中羟基红花黄色素A的定量鉴别方法.采用 25%甲醇水溶液提取、0.25 mL/min流动相流速、5 μL进样体积、40℃柱温箱温度、以甲醇-10 mmol/L乙酸铵水溶液为流动相、梯度洗脱,在多反应监测模式下使用铂金埃尔默色谱柱C18(2.7 μm,2.1mm×100 mm)对样品进行分析.结果表明:该方法的质量浓度在 0.02～2.00 mg/L时具有良好的线性关系,且拟合度大于 0.999 0;最低检出限为 0.125 μg/L,定量限为 0.42 μg/L,回收率在不同质量浓度下为 80%～95%;该方法能够为羟基红花黄色素A的判定提供高效灵敏的检测方案,也为天然染料染色纺织品的产品质量判定提供参考依据.
Abstract
In order to explore the identification method of hydroxyl safflower yellow A in natural dyes,which is distinguished from chemical synthetic dyes,a method for the quantitative analysis of hydroxyl safflower yellow A in natural dyes was developed by high performance liquid chromatography-tandem mass spectrometry.The samples were qualitatively and quantitatively analyzed in the multi reaction monitoring mode(MRM),25%methanol aqueous solution was used for extraction,the flow rate of mobile phase was 0.25 mL/min,the sample volume was 5 μL,the column temperature was 40℃,and methanol-10 mmol/L ammonium acetate aqueous solution was used as mobile phase,then separated by Perkin Elmer column C18(2.7 μm,2.1 mm×100 mm)The results show that the linear relationship is good when the mass concentration is 0.02-2.00 mg/L,and the fitting degree is higher than 0.999 0.The limit of detection was 0.125 μg/L,and the limit of quantitation was 0.42 μg/L,and the recovery was between 80%and 95%at different concentrations;This method can provide an efficient and sensitive detection scheme for the determination of hydroxyl safflower yellow A,and also provide a reference for the product quality determination of natural dye-dyed textiles.
关键词
羟基红花黄色素A/高效液相色谱串联质谱法/定量分析/检出限/回收率Key words
hydroxy safflower yellow A/high-performance liquid chromatography-tandem mass spectrometry/quantitative analysis/detection limit/recovery引用本文复制引用
出版年
2024
NETL
NSTL
万方数据