首页|3D-printed Mg-1Ca/polycaprolactone composite scaffolds with promoted bone regeneration
3D-printed Mg-1Ca/polycaprolactone composite scaffolds with promoted bone regeneration
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维普
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In bone tissue engineering,polycaprolactone(PCL)is a promising material with good biocompatibility,but its poor degradation rate,mechani-cal strength,and osteogenic properties limit its application.In this study,we developed an Mg-1Ca/polycaprolactone(Mg-1Ca/PCL)composite scaffolds to overcome these limitations.We used a melt blending method to prepare Mg-1Ca/PCL composites with Mg-lCa alloy powder mass ratios of 5％,10％,and 20％.Porous scaffolds with controlled macro-and microstructure were printed using the fused deposition modeling method.We explored the mechanical strength,biocompatibility,osteogenesis performance,and molecular mechanism of the Mg-1Ca/PCL com-posites.The 5％and 10％Mg-1Ca/PCL composites were found to have good biocompatibility.Moreover,they promoted the mechanical strength,proliferation,adhesion,and osteogenic differentiation of human bone marrow stem cells(hBMSCs)of pure PCL.In vitro degradation experiments revealed that the composite material stably released Mg2+ions for a long period;it formed an apatite layer on the surface of the scaffold that facilitated cell adhesion and growth.Microcomputed tomography and histological analysis showed that both 5％and 10％Mg-1Ca/PCL composite scaffolds promoted bone regeneration bone defects.Our results indicated that the Wnt/β-catenin pathway was involved in the os-teogenic effect.Therefore,Mg-1Ca/PCL composite scaffolds are expected to be a promising bone regeneration material for clinical application.Statement of significance:Bone tissue engineering scaffolds have promising applications in the regeneration of critical-sized bone defects.However,there remain many limitations in the materials and manufacturing methods used to fabricate scaffolds.This study shows that the devel-oped Ma-1Ca/PCL composites provides scaffolds with suitable degradation rates and enhanced bone formation capabilities.Furthermore,the fused deposition modeling method allows precise control of the macroscopic morphology and microscopic porosity of the scaffold.The obtained porous scaffolds can significantly promote the regeneration of bone defects.
3D printingBone tissue engineeringMagnesiumOsteogenicPolycaprolactoneScaffold
Xiao Zhao、Siyi Wang、Feilong Wang、Yuan Zhu、Ranli Gu、Fan Yang、Yongxiang Xu、Dandan Xia、Yunsong Liu
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Department of Prosthodontics,Peking University School and Hospital of Stomatology,Beijing 100081,China
National Center of Stomatology,National Engineering Laboratory for Digital and Material Technology of Stomatology,National Clinical Research Center for Oral Diseases,Beijing Key Laboratory of Digital Stomatology,Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health,NMPA Key Laboratory for Dental Materials,Beijing 100081,China
Department of Dental Materials,Peking University School and Hospital of Stomatology,Beijing 100081,China
维普
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